Phellsgate 2

Author: jdux

August undefined, 2024

http://www.biofeng.com/zaiti/zhiwu/pHELLSGATE.html WebpHELLSGATE 12 High-throughput binary Gateway® vector with two introns, for silencing genes in plants using intron-containing hairpin RNA. Open in SnapGene Try SnapGene for …

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WebPhellsgate 2 Vector Thermo Fisher Bioz Bioz Stars score, Techniques, Protocol Conditions and more for Phellsgate 2 Vector, supplied by Thermo Fisher. Data for Phellsgate 2 … WebpHELLSGATE is an efficient, high-throughput and effective plant gene silencing vector. Vector Type. Gene Silencing Vector, Gateway Vector, Plant Dual Expression Vector ... Powder; Solution Size. 2 μg; 10 μL . High Copy/Low Copy. High Copy . Prokaryotic Resistance. Spectinomycin . Length. 18691 bp . Selectable Marker. fastboot tool huawei

pHELLSGATE 12 Sequence and Map - SnapGene

WebOverwatch 2 Elden Ring Genshin Impact GTA Reviews Giveaways Videos Free Halo x Portal Shooter Coming to Consoles In August. Share Facebook Twitter Reddit WhatsApp Copy … Web... original pHellsgate vector (Wesley et al. 2001) and pHellsgate 4 ( Fig. 1) were designed to facilitate a one-step cloning process where a single PCR product flanked by attB1 and … WebIn this paper we describe two improved pHellsgate vectors that facilitate rapid generation of hpRNA-encoding constructs. pHellsgate 4 allows the production of an hpRNA construct in … freie cad software architektur

pHELLSGATE 8 vector map and sequence - novoprolabs.com

A high-throughput inducible RNAi vector for plants

WebSep 9, 2024 · PAL1 gene was amplified from cassava cDNA using primers with 5′ adaptor (attB) sequences and cloned into pHELLSGATE vector (CISRO, Australia) using Gateway cloning to prepare the RNAi construct (Additional file 1: Table S1) . pHELLSGATE-PAL1 RNAi construct was transformed into competent cells of the super virulent Agrobacterium … WebpHELLSGATE, pWATERGATE, pSTARGATE and pOpOff contain Gateway TM recombination sites. Gateway TM is proprietary technology owned by Invitrogen and to the extent that CSIRO vectors incorporate Gateway TM technology, ... 5.2 ALL TERMS, CONDITIONS AND WARRANTIES (INCLUDING ANY WARRANTY AS TO MERCHANTABLE QUALITY OR … fastboot to recoveryWebApr 5, 2024 · [0072] 实施例4：mswrky41基因转化紫花苜蓿的获得 [0073] 农杆菌准备：将在-80℃冰箱中保存的phb-mswrky41-flag和phellsgate-mswrky41rnai农杆菌菌株分别在含有抗生素为kan50+rif100和spe50+rif100的yeb固体培养基上划线活化，28℃培养48h后，挑取单克隆至含有相同抗生素的yeb液体培养 ... fastboot tools download

"Web2. Why did you need pART27 for? It is a binary vector, people use it for Agrobacterium transformation for introducing RNAi into plants (see attached paper). 3. I attached the original paper... " - Phellsgate 2

Phellsgate 2

Constructs and Methods for Hairpin RNA-Mediated Gene

WebDOI: 10.1111/j.1467-7652.2005.00149.x Abstract We describe here a vector system that allows dexamethasone-inducible RNAi against plant genes. The system utilizes a modified pHELLSGATE vector, under the control of the pOp6 promoter, and … WebAfter inoculation with F. solani for 72 h, the PnPR-like expression level in the control leaves was about 5-times higher than that in the leaves transformed with pHellsgate 2-PnPR-like, which ...

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Web豆丁网是面向全球的中文社会化阅读分享平台，拥有商业,教育,研究报告,行业资料,学术论文,认证考试,星座,心理学等数亿实用 ... WebJan 16, 2014 · Recombinations reaction between the pDONOR221-attB-PHYA1 and the pHellsgate-8 vector with 35S promoter 11 were conducted in a total volume of 10 μl, with 2 μl LR clonase buffer (Invitrogen), 2 ...

WebBioz Stars score, Techniques, Protocol Conditions and more for Binary Vector Phellsgate 2, supplied by Thermo Fisher. Data for Binary Vector Phellsgate 2 gathered from related … Web(Invitrogen), and 150 ng plasmid of pHellsgate 2. The reaction was held at room temperature (25 C) for 4 h. Thereafter, 1 lL proteinase K was added and incubated at 37 C for 10 min to stop the reaction. About 2 lL mix was used to transform the Escherichia coli DH10B. The plas-mids of positive clones on the plate with spectinomycin

WebpHELLSGATE 12 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available … WebJan 2, 2015 · Cinnamyl AlcoholDehydrogenase (CAD) is the final enzyme involved in the monolignol biosynthesispathway.Transgenic poplars were produced using RNAi strategy (pHellsgate 8 vector, Helliwell and Waterhouse, 2003) and exhibited the expectedphenotype (red xylem, reduced CAD activity). -RNAi-CAD transgenic poplars were produced using …

WebpHELLSGATE载体价格10000元，pHELLSGATE载体质粒图谱(Vector map)，载体序列(Sequence)见下文，载体质粒抗性壮观霉素或链霉素，质粒大小为18691BP，测序引物见 … freie fachinformationenWebThe system utilizes a modified pHELLSGATE vector, under the control of the pOp6 promoter, and the synthetic transcription factor, LhGR. We demonstrate that the production of RNAi … fastboot to recovery modeWebBriefly, the partial fragments of FaSAUR1 and FaSAUR2 (Supplementary Figure S5) were ligated into pHELLSGATE 2 vector (Shi et al., 2024) using BP Clonase (Invitrogen, MA, USA) to construct 35Spro... freie downloadsWebJan 13, 2024 · sequence as well as the RNAi vector pHellsgate 2 were subjected to a BP recombination reaction using the BP Clonase TM II enzyme mix (Invitrogen, United States). freie liebe alligatoah lyricsWebApr 8, 2024 · Zestimate® Home Value: $295,000. 902 Falgate Ct, Windy Hills, KY is a single family home that contains 2,200 sq ft and was built in 1979. It contains 4 bedrooms and 2 … freie downloads bilderWebAug 1, 2015 · C12N15/825 Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic... freie fachinformationen gmbhpHELLSGATE High-throughput binary Gateway® donor vector for silencing genes in plants using intron-containing hairpin RNA. Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7k Plasmids: Plant Vectors More Plasmid Sets Home Plasmids Plant VectorspHELLSGATE Show Static Map fastboot tool for android