WebAntibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. WebThis antibody recognizes tyrosine-phosphorylated proteins from all species. Immunogen Phosphotyramine-KLH Application Anti-Phosphotyrosine Antibody, clone 4G10 detects …
Anti-Phosphotyrosine Antibody, clone 4G10 ® , Biotin Conjugate
WebSen Chen's publication in Sci Signal. ...against pTyr (4G10) and tubulin were from Millipore, and antibody against pMET (Tyr1234) was from GeneTex. Bmxtides were from … WebAnti-Phosphotyrosine Antibody, clone 4G10 detects tyrosine phosphorylated proteins in all species. This unique monoclonal antibody is validated for use in IC, IH, IP, WB and is … how to show artboard in illustrator
Anti-Phosphotyrosine Antibody, clone 4G10® 05-321X - Merck …
WebMar 23, 2024 · The membranes were afterward incubated with mouse anti-phosphotyrosine antibody (clone 4G10; Millipore, MA, USA) diluted 1:1,000 in PBS or mouse anti-ubiquitin antibody (FK2, 1:250 dilution, monoclonal mouse antibody recognizing mono- and polyubiquitinated conjugates; ENZO Life Sciences, NY, USA) at 4°C overnight. After … WebApr 12, 2024 · To measure the protein phosphotyrosine and acrosine availability, cell aliquots were fixed, permeabilized and blocked as described above (section actin polymerization). Subsequently, the samples were incubated for 1 h with specific anti-phosphotyrosine (4G10 Platinum, Sigma‒Aldrich) or anti-acrosine (ACR-2, Exbio, Praha, … WebImmunoblot analysis comparing untreated GST-Abl kinase to dephosphorylated GST-Abl kinase was routinely done using phosphotyrosine-specific antibody 4G10 to confirm complete (>95%) dephosphorylation of tyrosine residues and c-Abl antibody CST 2862 to confirm equal loading of GST-Abl kinase. The inhibitor concentration ranges for IC50 ... nottingham psychics