Pulk1 s757
WebDec 6, 2024 · Reactivation of mTORC1 in full medium within 1 h was observed by phosphorylation of downstream substrates pULK1 (S757), pWIPI2 (S413), and pAMBRA1 (S52), ... Interestingly, although we observed reduced ULK1 S757 phosphorylation in USP32 KO cells and also accumulation of nuclear TFEB as a consequence of reduced mTORC1 … WebpULK1(S757)(CS6888),rabbitanti-mTOR(CS2972),andrab-bit anti-phospho-mTOR (S2448) (CS2971S) from Cell Signal-ingTechnologyandmouseanti- -actin(sc-47778)fromSanta Cruz Biotechnology. Secondary antibodies were donkey anti-rabbit IRDye 680RD and donkey anti-mouse IRDye 800CW from LiCor. Blots were stripped between incubations using
Pulk1 s757
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WebJan 24, 2024 · Besides, pAMPK T172 and pULK1 S556 were decreased, and pmTOR S2448 and pULK1 S757 were increased. Cell experiments showed that the level of … WebBoster Bio Anti-ULK1 (S757) Antibody catalog # A00584S757. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody.
WebMay 19, 2013 · Whole-cell lysates were immunoblotted with pULK1 S757 (mTORC1-target site), pS6K and ULK1 antibodies (bottom panels). Two unique experiments were performed. WebAug 11, 2015 · pULK1 (S757) pS6K (T389) S6K Rictor pRictor (T1135) pS6 (S235/236) B-- - MK PF R AS160 Akt pathway mTORC2 pathway mTORC1/S6K pathway C pSIN1 (T86) pAkt (T308) pS6 (S235/236) pNDRG1 (T346) 14-3-3 pPRAS40 (T246) Insulin Inhibitor PRAS40 Protor mTORC1 mTOR Raptor mLST8 Growth Protein Synthesis S6K …
WebNational Center for Biotechnology Information WebpULK1-S555. Data are shown as mean phosphorylation (arbitrary units) ± s.e.m.; n = 3. Statistical significance vs. vehicle-treated WT was calculated by one-way ANOVA with Dunnett’s multiple-comparisons test. Representative immunoblots are shown. C) HEK293T cells expressing FLAG-tagged a1b1g1 or a2b1g1 were treated with 250 nM rapamycin or
WebJun 29, 2024 · Detection of pULK1 (S757) (A) or LC3-I/LC3-II (B) in B cells infected with indicated Salmonella strains for 3 h. ( C ) CFUs recovered from B cells infected with Salmonella strains for 24 h and treated for 1.5 h with autophagy-inducer peptide D11(20 μM) or scramble peptide L11 (20 μM).
WebSep 17, 2024 · Interestingly, ULK1 S757 phosphorylation was not only maintained, ... (Figs. 2 and 6), signs of an mTORC1-dependent autophagy deficiency such as pULK1 S757 might also signal metabolic susceptibility. phone number for frame shop in live oak flWebPolyclonal Antibody for studying ULK1 (Ser758) phosphate. Cited in 342 publications. Validated for Western Blotting. Available in 2 sizes. Highly … phone number for frederick news postWebFeb 1, 2024 · Western blot analysis exhibits the effect of Alda-1 on the phosphorylation of ULK1 with high glucose exposure. G: Representative gel blots of pULK1and β-actin … how do you put a fire outWebNov 25, 2024 · As mentioned above, we confirmed the autophagy activation signaling by measuring pAMPK (T172), pULK1 (S757), pULK1(S555), pS6 (S235/236) and Atg5-12 conjugation. When mice were treated with HCQ to block autophagy flux, besides p62 and LC3II accumulation, we also observed that Atg5-12 conjugated form was increased in the … phone number for ftx usWebAug 28, 2024 · The densities of p-ATG13, p-BECN1, and p-ULK1 S757 are quantified and showed in the right panel. f HeLa cells were treated with different concentrations of PKC … phone number for foxwoods casinoWebPulk1 S757, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more how do you put a fingertip bandagesWebEtOH altered ULK1 S555 and S757 phosphorylation in a time- and AMPK-dependent manner. The activation of AMPK and ULK1 was associated with increased BECN1 (S93, S14) and PIK3C3/VPS34 (S164) phosphorylation as well … how do you put a fraction into a decimal